LATEST NEWS AND UPDATES

Ubiquitome one of Bill Reichert’s top 10 businesses
Featured in his report, he says his jaw dropped when he heard the Ubiquitome story of developing “what may be the platform that enables genetic analysis to become a ubiquitous standard diagnostic device.”
Read more.
Analysis in the field – the impact of real-time PCR testing
In 2015, Holly Bowers was a finalist in the Freedom For You grants program. Her prize was a Freedom4 device, which allowed Holly to perform real-time analysis in the field for different algal bloom species.
Read more.

Evaluation of the Freedom4 - a mobile real-time PCR device for robust detection of Zaire ebolavirus

Comparable performance by the Freedom4, a mobile real-time PCR device, to the QuantStudioDx (Life Technologies) was demonstrated in these experiments. It is concluded that the Freedom4 gives the same performance and sensitivity as in laboratory instruments.

Application notes

Performance of the Freedom4 for detection of Pseudo-nitzschia australis
Performance of the Freedom4 for detection of Batrachochytrium dendrobatidis
A high impact role for the Freedom4 device in Forest Protection

APPLICATIONS

Click on each application to see how the Freedom4 can help researchers in their respective fields.
Clinical
"Early diagnosis of HIV among infants born to HIV-positive mothers is a critical component of prevention of mother-to-child transmission of HIV."
Joseph Mwangi
Kenya Medical Research Institute, Nairobi
Animal Health
"By having a rapid on site (poultry farm and / or slaughterhouse) test available, it allows rapid discriminate between Campylobacter contaminated flocks and non-contaminated high quality flocks."
Jos van der Vossen
TNO
Food
"Compared to cumbersome and costlier ELISA method, the PCR technique is simple and allows us to estimate the quantitative levels of the IYSV nucleoprotein (N) gene transcripts in infected plants."
Diwaker Tripathi
Washington State University
Environment
“Currently we have boat days where we bring back whole water to shore and use the microscope to assess whether our bug of interest is there or not."
Holly Bowers
Monterey Bay Aquarium Research Institute
Conservation
"At the moment, it is virtually impossible to get samples and swabs out of Latin America as their biosecurity laws have tightened up immensely. This means the need for testing abilities on the ground is paramount."
Robert Puschendorf
School of Biological Sciences, Plymouth University
Forensics
"The ability to perform real-time PCR at crime scenes for the detection and identification of body fluids would be a significant advantage in crime scene investigations." 
Rachel Fleming
Institute of Environmental Science and Research Ltd

PERFORMANCE

The Freedom4 has been designed to meet or exceed the performance of laboratory based real time PCR platforms.

“All established assays were shown to be transferable and gave comprehensible results .”
- The Institute of Environmental Science and Research (ESR)

SPECIFICATIONS

INSTRUMENT
Block Format 4-well block
Thermal system Peltier-based system
Consumables Low profile, high thermal performance plates
Sample volume 10μl to 40μl
Average ramp rate 2.3°C/sec
Temperature range 120°C Maximum/0°C Minimum
Ambient operation 10°C – 35°C
Optical system Single laser diode (473nm) and photodiode array
Calibrated dyes SYBR Green, FAM
Data collection Data collected for all wells regardless of plate setup.
Real-Time PCR run time (40 cycles) 50 minutes
DIMENSIONS
Outside dimensions Length closed: 20 cm (7.87 in)
Length open: 24 cm (9.44 in)
Width: 10 cm (3.94 in)
Height: 8.5 cm (3.34 in)
Weight 2.4 kg (5.29 lb)

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